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| Sat 21-09 | Regional 1 Midlands 2024-25 | Kenilworth | Bromsgrove 1 | Ref: Josh Bate |
| Sat 21-09 | Regional 1 North East 2024-25 | Alnwick 1 | Blaydon 1 | Ref: Andrew Shaw |
| Sat 21-09 | Regional 1 North East 2024-25 | Percy Park 1 | Ilkley 1 | Ref: Jonny Cartwright |
| Sat 21-09 | Regional 1 North West 2024-25 | Birkenhead Park | Rossendale 1XV | Ref: Adam Tweddle |
| Sat 21-09 | Regional 1 North West 2024-25 | Kendal 1 | Blackburn 1XV | Ref: Matt Arrowsmith |
| Sat 21-09 | Regional 1 North West 2024-25 | Manchester 1XV | Penrith 1 | Ref: Harry Groves |
| Sat 21-09 | Regional 1 North West 2024-25 | Stockport 1XV | Wirral | Ref: Mark Hyde |
| Wed 25-09 | BUCS Premier South 1 Men. 2024-25 | Hartpury University II | Brunel University Mens 2s | Ref: Matt Arrowsmith |
| Sat 28-09 | Regional 1 North East 2024-25 | Percy Park 1 | Alnwick 1 | |
| Sat 28-09 | Regional 1 North West 2024-25 | Anselmians | Kendal 1 | |
| Sat 28-09 | Regional 1 North West 2024-25 | Blackburn 1XV | Birkenhead Park | |
| Sat 28-09 | Regional 1 North West 2024-25 | Rossendale 1XV | Stockport 1XV | |
| Sat 28-09 | Regional 1 North West 2024-25 | Wirral | Leek | |
| Wed 02-10 | RFU AASE North Group 2024-25 | Gosforth Academy | City of Oxford College | Ref: Anthony Lowery |
| Wed 02-10 | RFU AASE North Group 2024-25 | Myerscough College 1XV | Bishop Burton College U18 1 | |
| Sat 12-10 | Regional 1 North East 2024-25 | Alnwick 1 | Cleckheaton 1 | |
| Sat 12-10 | Regional 1 North East 2024-25 | Blaydon 1 | Huddersfield 1 | |
| Sat 12-10 | Regional 1 North West 2024-25 | Birkenhead Park | Anselmians | |
| Sat 12-10 | Regional 1 North West 2024-25 | Kendal 1 | Stoke On Trent | |
| Sat 12-10 | Regional 1 North West 2024-25 | Manchester 1XV | Newport (Salop) 1 | |
| Sat 12-10 | Regional 1 North West 2024-25 | Penrith 1 | Wirral | |
| Sat 12-10 | Regional 1 North West 2024-25 | Stockport 1XV | Blackburn 1XV | |
| Sat 12-10 | Regional 1 South Central 2024-25 | London Welsh Amateur 1 | Tunbridge Wells | |
| Sat 12-10 | Regional 1 South Central 2024-25 | Wimbledon 1 | Hammersmith & Fulham 1 | |
| Wed 16-10 | RFU AASE North Group 2024-25 | Gosforth Academy | Bishop Burton College U18 1 | |
| Wed 16-10 | RFU AASE North Group 2024-25 | Myerscough College 1XV | Oaklands College 1 (Wolves) | |
| Sat 19-10 | Regional 1 North East 2024-25 | Percy Park 1 | Scunthorpe I | |
| Sat 19-10 | Regional 1 North West 2024-25 | Anselmians | Stockport 1XV | |
| Sat 19-10 | Regional 1 North West 2024-25 | Blackburn 1XV | Leek | |
| Sat 19-10 | Regional 1 North West 2024-25 | Kendal 1 | Manchester 1XV | |
| Sat 19-10 | Regional 1 North West 2024-25 | Rossendale 1XV | Penrith 1 | |
| Sat 19-10 | Regional 1 North West 2024-25 | Wirral | Newport (Salop) 1 |